移动式叶绿素荧光成像系统

移动式叶绿素荧光成像系统PlantExplorer^XS是由慧诺瑞德和荷兰PhenoVation公司联合推出的专门针对大田、温室、气候室和实验室场景的可以移动的叶绿素荧光测量系统。配备移动式升降平台车、内置电脑的叶绿素荧光成像单元、移动电源、显示单元和操作单元。叶绿素荧光成像单元可以升降和旋转，既可以测量不同高度的植物冠层，也可以倾斜或水平角度测量穗（麦穗、稻穗、谷穗等）、荚果（大豆、油菜等）、果实（番茄、黄瓜、葡萄、柑橘等）、叶片或冠层。

该系统成像面积为18x18cm，具备500万像素高清成像，同时具备“调制”和“非调制”叶绿素荧光成像测量功能，既可以测量光合生理，也可以测量形态结构，同时配备功能强大的控制和分析软件，且可以对大量数据进行批处理分析。该系统，无论室内还是大田，都是进行植物表型、光合生理、植物抗逆、植物病理、育种、功能基因组、突变株筛选、种子生理/病理等研究的利器。
 

功能特性

• 大田、温室、气候室、实验室进行移动式测量
• 叶绿素荧光成像单元可以升降、旋转
• 叶绿素荧光成像和表型分析同步测量
• 同时具备调制和非调制叶绿素荧光测量功能
• 出色的高清相机(500万像素)、高信噪比成像
• 16位图像格式，的成像质量
• 光源、相机、滤光片、电脑一体化设计
• 无可见镜头畸变，无需图像校正
• 成像范围18 x 18cm
• 多种测量protocol可选，允许用户编辑设定自己的protocol，包括但不限于Fv/Fm测量、标准诱导曲线测量、暗弛豫测量、OJIP快速诱导动力学测量等等。
• 可进行功能强大的延时成像测量
• 自动计算荧光参数和表型参数
• 具备图像数据批处理分析功能
• 提供多种功能强大的图像分割功能
• 对所有图像数据均提供数据分级(用户自定义范围)并进行图像化显示，并允许对分级筛选后的数据叠加到可见光图像上展示
• 图像背景、伪彩色标尺均有多种选择
• 允许用户自定义多种ROI(性状、数目、分布等)并对ROI的数据自动分析
• 嵌入式电脑进行精确的成像、时间控制、光强控制和数据存储
• 功能强大的控制和分析软件
• 特别适合突变株筛选、育种材料/组合筛选、抗逆研究、病理研究、种子研究、果实研究、功能基因组学等

主要技术参数

• 基本组成：移动式升降平台、叶绿素荧光成像单元、移动电源、显示单元、操作单元等
• 叶绿素荧光成像方式：“调制”测量 +“费调制”测量
• 调制测量光：蓝色LED， 450nm，半峰全宽20nm，光强4000 umol m^-2 s^-1 ，独立触发
• Kautsky测量光：蓝色LED， 450nm，半峰全宽20nm，光强4000 umol m^-2 s^-1
• 饱和脉冲：蓝色LED， 450nm，半峰全宽20nm，光强4000 umol m^-2 s^-1，独立触发
• 时间分辨动力学光化光：红光LED，660nm，光强800 umol m^-2 s^-1
• 远红光：LED，735nm，半峰全宽20nm，35W
• 相机：CMOS传感器，500万像素
• 颜色深度：12bit
• 标准帧率：37.5 FPS
• 图像格式：16bit
• 相机光谱范围：400～1000 nm
• 接口：3个USB3.0，1个以太网口，1个HDMI接口
• 嵌入式电脑：4核处理器，8G内存，256G固态硬盘
• 成像面积：18cm x 18cm
• 升降高度：0-1200mm（高度可定制）
• 旋转角度：-90° ~ 90°
• 显示单元：15.6寸触摸显示屏
• 供电：35万mAh移动电源，额定容量1260Wh，峰值功耗1000W，待机功耗35W
• 系统尺寸：600mm x 720mm x 2000mm(长x宽x高）

测量参数

• 调制叶绿素荧光参数：Fo、Fm、Fv/Fm、dFq/Fm=DF/Fm、Fs’、Fm’、Fo’、Fq’/Fm’=Fv’/Fm’、rETR、NPQ、Y(NO)、Y(NPQ)、qN、qP、qL、1-qP和1-qL等;
• 非调制叶绿素荧光参数：Fo、Fi、Fm、1-Fi/Fm、IC-Area、IC-Area/Fv、PI、Rfd、dRfd、RfdFm和RfdFt等;
• 表型参数：(植物、种子、果实的)数目、轮廓面积、长度、宽度、凸包点数、凸包面积、凸包面积/轮廓面积、最小外接圆(质心、半径、面积)、最小外接矩形(长、宽、面积、角度、alpha)和骨架等。

利用PhenoVation叶绿素荧光成像技术发表的部分文献

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